Activated Carbon Cadmium

Sample details Pages: 17 Words: 5008 Downloads: 7 Date added: 2017/06/26 Category Statistics Essay Did you like this example? Introduction Environmental pollution by metals is a serious threat today due to increased industrial revolution and has become extensive. The current worldwide mine production of various heavy metals such as Cu, Cd, Pb, and Hg is very high (Pinto et al., 2004). These heavy metal sources include a number of diverse anthropogenic sources (textile, tannery and other industrial effluents, industrial solid wastes, urban runoff, sewage treatment plants, boating activities, agricultural fungicide runoff, domestic garbage dumps, and also by mining operations). Don’t waste time! Our writers will create an original "Activated Carbon Cadmium | Sciences Dissertations" essay for you Create order Metal toxicity and its effect on the growth and metabolism in higher plants is a subject that has a wide economical and ecological interest and also have been widely reviewed and gaining importance on several occasions over the last few decades (Brown and Jones, 1975; Foy et al., 1978; Ernst et al., 1992; Das et al., 1997; SanitÃÆ'Â ¡ di Toppi and Gabrielli, 1999; Hall, 2002; Clemens et al., 2002). Another interesting reason for this is out of the ninety naturally occurring elements fifty-three are heavy metals (Weast, 1984). Few metals are found to be essential for growth of plants, while few are found to be toxic for the growth of plants. Some of the heavy metals dont interact with the plant metabolism directly but decreases the level of soil microbes, affecting the growth of the plants (Niess, 1999). However, the accumulation in the soil has become a worldwide problem leading to the loss of agricultural productivity (Hasan, 2007). Among the heavy metals Cd is of special concern because of its potential toxicological profile even at lower concentration (Das Samantray, 1997). Cadmium is a toxic trace pollutant for humans, animals and plants, which enter the environment from various industrial processes, phosphate fertilizers and other anthropogenic activities, which then enters the food chain (Wagner, 1993). It has been estimated that more than 45% of all cadmium is used in electroplating, and that of 10% of total industrial cadmium use ends up in wastewater streams. Interests in the ecological effects of heavy metals has been growing recently, and assessing the phytotoxicity of cadmium is one of the most important criteria being used to avoid environmental risks, before these effluents can be recycled back for agricultural irrigation after some remediation or treatment processes. So it is inevitable that we should cope with the environmental damages induced by increased concentration of cadmium. The removal of cadmium (II) from wastewater stream therefore is of great importance as it can directly contaminate the ground water, aquatic organisms, agricultural lands and also the productivity. The global threat of reduction of uncontaminated ground water is also increasing exponentially with the scientific, industrial and technological developments (Tchobanoglous et al., 1991). Although various other techniques such as precipitation are most commonly used, to clean up the Cd contaminated water, the drawbacks of using those techniques, increases the need for an alternative technique which is more effective, reliable and also economically viable (USEPA, 1984). Cadmium being a highly toxic metal pollutant of soil inhibits root and shoot growth and yield production, affects nutrient uptake and homeostasis, and is frequently accumulated by agriculturally important crops including soybean with a significant potential to impair animal and human health (Sanita di toppi and Gabrielli, 1999). In plants such as pea, a number of toxic effects of Cd on metabolism has been reported, such as decreased uptake of nutrient elements (Sandalio et al., 2001), inhibition of various enzyme activities (Obata et al., 1996) and induction of oxidative stress (Lozano-Rodriet al., 1997, Romero-Puertas et al., 1999, Sandalio et al., 2001) including alterations in enzymes of antioxidant defense system (Dalurzo et al., 1997, Sandalio et al., 2001). However only little is known about the biochemical mechanisms underlying the intraspecific variation in the sensitivity versus tolerance to Cd in plants (Ashraf et al., 2005). Understanding the connections between a plants initial responses and the downstream events that constitute successful adjustment to its altered environment is one of the next grand challenges of plant biology (Ruth Grene, 2002). One of the common characteristics of these heavy metals such as cadmium is the induction of oxidative stress generating active oxygen species (AOS). Plants have evolved various protective defense mechanisms to eliminate or reduce these AOS species. One of them is the enzymatic or non-enzymatic antioxidant system, including peroxidase, Super oxide dismutase, catalase, glutathione peroxidase etc. Each of these parameters has physiological function under non-stressed conditions, but their activity or quantity is increased under oxidative stress (Sara Erdei, 2002). This study becomes more important because legume crops are less tolerant to Cd toxicity than cereals, grasses and frequently encounter strong inhibition of biomass production even at the less than micro molar range of cadmium (Inouhe et al., 1994). No much of the studies have been reported in the effect of cadmium in reproductive stage of soybean plant. Especially studies on the response of plants can very much help in increasing productivity, improving biomass, and reducing the green house gases, and also in the identification of Cd tolerant plants. Research studies on Cd effect on the economically important soybean crop may also be helpful to meet the increasing demand for soybean in India. The increasing global demand for soybean, oil and other products, rise in price at the international level particularly after the usage of soybean for biodiesel production, made us to select soybean as the plant of our research interest in this study. With the increasing scarcity for water and increasing pollution of ground water the irrigation of the legumes has become a big problem, in addition to the contaminated and less fertile soil for the farmers. Hence it is very essential to derive mechanisms to clean up technologies to remove the contaminants form wastewater and reuse of it for irrigation of legumes. Activated carbon absorption has been used successfully for the advanced treatment of municipal and industrial wastewater. Adsorption occurs when molecules adhere to the internal walls of pores in carbon particles produced by thermal or acid chemical activation (US EPA, 2000). Production of activated carbon from an easily available agro waste makes this technique more apt to adapt in a large-scale level. Also the reduction in the production cost by modification of already available techniques is very important and makes this process economically very feasible. Hence in our study we have selected the agricultural waste, shell from Anacardium occidentale L., a member of the Anacardiaceaefamily, of which the cashew is by far the most important economically. Anacardium occidentale L., is cultivated commercially in 32 countries on over 7.5 million acres. Cashew is now the #1 tree nut crop in the world, since its production surpassed that of almond in 2003. Average yields worldwide are about 600 lbs/acre. And India ranks second in its production contributing to 25% of the total production worldwide. Anacardium occidentale L produce 2 additional products of commercial value from their fruit: cashew apples and cashew nut shell liquid. After the CNSL is taken, the left out biological waste shell is thus considered for the production of low cost activated carbon in our study, which is available in higher quantity at a very cheaper price. Hence this study may be increasing one more commercially important product from the waste of Anacardium occidentale L. Objectives of The Study The major objectives of this research project are Use of the left out agro waste shell of Anacardium occidentale L., for the production of low cost activated carbon, in turn increasing one more commercially important product from the waste of Anacardium occidentale L., this also may be helping to improve the production of activated carbon in India to meet the increasing global need in the activated carbon industry. Development of an activated carbon process as the cleaning technology for removing heavy metal cadmium, from the Cd contaminated water Reuse of the treated water for growing economically important legume soybean and studying the response of soybean grown with the cadmium contaminated water before and after treatment. Specific Aims The specific aims of this study are as follows Study of the effect of Cd on the growth, stress and antioxidant metabolism of Glycine max (L.) Merr. Plants by petriplate and pot culture studies. Production and characterization of low cost activated carbon from the agro waste nut shell of Anacardium occidentale L. Batch mode experiments for the optimizing the conditions for maximum removal of Cd by the activated carbon from the biological waste nut shell of Anacardium occidentale L. Application of the treated water for irrigation and study of effect of Cd contaminated water after treatment on the growth, stress and antioxidant metabolism of Glycine max (L.) Merr. Plants by petriplate and pot culture studies. Evaluation of the effect of treated water on the growth on the growth of soybean over the treated water. A study on uptake and localization of cadmium by various parts of soybean Experimental Design and Salient Findings The experimental protocols are deduced and the work is designed in order to fulfill the objectives of the study and was carried out in eight phases, following standard protocols wherever necessary. PHASE-I: Collection of Seeds, Soil and Biological Waste Sample A. COLLECTION OF SEEDS, SOIL AND AGRO WASTE: Co-2 variety soybean [Glycine max (L.) Merr] seeds were collected from Millets and New Area Farm, Centre for Plant Breeding and Genetics, Tamilnadu Agricultural University, Coimbatore, and used for the study. The biological waste of shell of Anacardium occidentale L.was collected and the activated carbon is produced by the acid activation process. B. PREPARATION OF SYNTHETIC EFFLUENTS: The synthetic effluents with concentrations varying from 5, 10, 15, 20 and 25 ppm concentration of cadmium were prepared by using cadmium chloride, by calculating the level of cadmium in CdCl2 and used for growing the plants during germination, pot culture studies and for Cd adsorption studies. Distilled water is used as the control for all the experiments. C. COLLECTION OF THE SOIL AND SOIL STUDY Red soil was collected from the agricultural fields of Coimbatore, the composition is checked, and used throughout the study. The soil parameters checked includes, Moisture (%), pH, Electrical conductivity, Total nitrogen, Total phosphorus, Total potassium, Organic carbon, C / N ratio, Available Nitrogen, Available Phosphorus, Available potassium, Exchangeable calcium, Exchangeable Magnesium, Exchangeable sodium, Calcium, Magnesium, Sulphate, Chloride, Cadmium. Findings: The above parameters were checked to ensure that the soil is suitable for the growth of soybean. The Cd level was determined using Atomic Absorption Spectroscopy and found to be below non detectable limit and hence used for the study. PHASE-II Effect of Cadmium On Germination of Soybean (Petriplate Study 10 Days) The plants were grown in six groups as follows, and five plants were selected for checking germination parameters. GROUP-I NORMAL WATER TREATED SOYBEAN GROUP-II 5 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-III 10 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-IV 15 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-V 20 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-VI 25 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN Parameters Checked: The parameters checked on 10th day includes Germination percentage, Root length, Shoot length, Length of whole plant, Fresh mass of whole plant, Dry mass of whole plant, Dry mass / fresh mass ratio, Relative seed germination, Root length, Shoot length percentage, Germination index, Seed vigor index, Tolerance index, Germination rate Percentage. Findings of Germination Study Germination of soybean., seedlings was drastically affected by the increasing concentration of cadmium in the effluents. A significant decrease in germination percentage, root length, shoot length, fresh mass, dry mass of whole plant, relative seed germination, root length, shoot length percentage, germination index, seed vigor index, tolerance index and germination rate percentage was observed. Also a significant increase in dry mass / fresh mass ratio was observed. With these preliminary results obtained the work was extended to red soil study using pot culture. PHASE -III Pot Culture Studies EFFECT OF CADMIUM ON SOYBEAN OBSERVED ON 20TH DAY AND 40TH DAY: The plants were grown in six groups as follows, and five plants were selected for checking all physical parameters and all biochemical estimations were made on three plants selected as replications. GROUP-I NORMAL WATER TREATED SOYBEAN GROUP-II 5 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-III 10 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-IV 15 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-V 20 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-VI 25 ppm Cd CONTAMINATED ARTIFICIAL EFFLUENT TREATED SOYBEAN III.A. GROWTH PARAMETERS CHECKED ON 20TH DAY The parameters checked on 20th day includes Root length, Shoot length, Plant height, Relative root length percentage, Relative shoot length percentage, Tolerance index, No of leaves, trifoliate, fresh weight, dry weight, Dry mass/fresh mass ratio, Leaf mass ratio, Stem mass ratio, Root mass ratio, Total dry matter yield under cadmium stress, Percentage weight gain, Seed vigor index, Leaf area measurements, Leaf area index, Leaf weight fraction, Specific leaf area, Leaf area ratio. FINDINGS: In the observations made with five plants in each group on 20th day, the following results were observed. The growth parameters such as root length, shoot length, plant height, relative root length percentage, relative shoot length percentage, tolerance index, no of leaves, trifoliates, fresh weight, dry weight were found to be decreased significantly with increasing concentrations of cadmium level in the effluents when compared to control. Also dry mass/fresh mass ratio, stem mass ratio, root mass ratio were found to be increased, while the other parameters such as leaf mass ratio, total dry matter yield under cadmium stress, percentage weight gain, seed vigor index, leaf area measurements, leaf area index, leaf weight fraction, specific leaf area, leaf area ratio were also found to be decreasing when compared to control group plants, with increasing levels of cadmium in the effluents. III.B. GROWTH PARAMETERS CHECKED ON 40TH DAY The parameters checked on 20th day includes Root length, Shoot length, Total plant height, Relative root length percentage, relative shoot length percentage, tolerance index, Number of leaves, trifoliate, nodules per plant, fresh weight, dry weight of individual organs, and whole plant, Dry mass/fresh mass ratio of individual organs, Leaf mass ratio, Stem mass ratio, Root mass ratio, Total dry matter yield under cadmium stress, Percentage weight gain, Seed vigor index, Leaf area measurements, Leaf area index, Leaf weight fraction, Specific leaf area, Leaf area ratio, Absolute growth rate, Crop growth rate, Unit leaf rate, Net assimilation rate, Relative growth rate, Leaf area duration FINDINGS: In the observations made with five plants in each group on 40th day, the following results were observed. The growth parameters such as root length, shoot length, plant height, relative root length percentage, relative shoot length percentage, tolerance index, number of leaves, trifoliates, nodules per plant, fresh weight, dry weight of individual organs, and whole plant, leaf mass ratio, total dry matter yield under cadmium stress, percentage weight gain, seed vigor index, leaf area measurements, leaf area index, leaf weight fraction, specific leaf area and leaf area ratio were found to be decreased significantly. The dry mass/ fresh mass ratio, stem mass ratio and root mass ratio were found to be increasing with increase in the concentration of cadmium. The growth parameters connecting the observations made on 20th and 40th day observations such as absolute growth rate, crop growth rate, unit leaf rate, net assimilation rate, relative growth rate, leaf area duration values showed a significant difference from the values of control group plants. III.C. BIOCHEMICAL PARAMETERS ESTIMATED ON 20TH DAY IN ROOT AND SHOOT TISSUES: The various biochemical estimations made includes, i. GROWTH PARAMETERS Chlorophyll a, b, total chlorophyll, Total carbohydrate, Starch, total Protein ii. ENZYMES: Amylase alpha, beta and total, Glucose-6-phosphate dehydrogenase iii. STRESS MARKERS Total free amino acids, Proline, TBARS iv. NON ENZYMATIC ANTIOXIDANTS Vitamin E, Vitamin C, Reduced Glutathione v. ENZYMATIC ANTIOXIDANTS Superoxide dismutase, Catalase, Peroxidase, Glutathione Reductase, Glutathione Peroxidase. FINDINGS: The levels of growth parameters such as Chlorophyll-a, b and total Chlorophyll were significantly found to be affected with the increase in the cadmium concentrations in the effluents. The levels of Total Carbohydrate, Starch and Total Protein were also estimated in root and leaf tissues and were found to be significantly decreased with increasing concentrations of cadmium. The decreasing levels of Amylase, Glucose-6-phosphate dehydrogenase were also observed with increasing concentrations of cadmium, when compared to control in both root and leaf tissues. The stress markers like Total Free Amino Acids, Proline and Thiobarbituricacid reactive substances (TBARS) were also found to be increased with increase in the concentrations of cadmium in both leaf and root tissues. The non enzymatic antioxidants such as Vitamin E, Ascorbic acid, and Reduced Glutathione were found to significantly varying with the varying concentrations of cadmium. The enzymatic antioxidants such as Catalase, Peroxidase, Glutathione Reductase, Glutathione peroxidase were found to be decreased in their levels with increasing concentration of cadmium. Superoxidedismutase was found to be increasing with increase in the Cd concentration. PHASE-IV PRODUCTION AND USE OF LOW COST ACTIVATED CARBON FOR REMOVAL OF CADMIUM FROM CONTAMINATED EFFLUENT ADSORPTION STUDIES. COLLECTION OF THE BIOLOGICAL WASTE. ACTIVATED CARBON PRODUCTION BY ACID ACTIVATION. PHYSICAL PROPERTIES OF ACTIVATED CARBON FTIR ANALYSIS OF ACTIVATED CARBON BEFORE ADSORPTION SEM ANALYSIS BEFORE ADSORPTION FINDINGS: The agricultural waste, shell from Anacardium Occidentale L., were collected, and the activated carbon is produced, the activated carbon of size less than 125 micron was selected and its physical parameters were determined, and is found to be suitable for removal of cadmium from waste water. Fourier Transform Infra Red spectroscopy and Scanning Electron Microscopy analysis were done for the prepared activated carbon. PHASE-V BATCH MODE ADSORPTION STUDIES Batch mode adsorption studies were done for the following parameters EFFECT OF EQUILIBRIUM TIME EFFECT OF pH EFFECT OF VARYING CARBON CONCENTRATION EFFECT OF VARYING CADMIUM CONCENTRATION EFFECT OF TEMPERATURE FINAL PERCENTAGE REMOVAL OF CADMIUM FROM VARIOUS CONC OF Cd SOLUTIONS. FTIR ANALYSIS OF ACTIVATED CARBON AFTER ADSORPTION SEM ANALYSIS AFTER ADSORPTION FINDINGS: The batch mode experiments were performed for adsorption studies and the optimum equilibrium time, optimum pH, effects of varying carbon concentration and varying carbon concentration and temperature were done and the carbon to cadmium ratio was determined. The final percentage of cadmium removed successfully after treatment with optimum concentrations was determined using Atomic Absorption Spectroscopy. The carbon collected after adsorption was analyzed using Fourier Transform Infra Red spectroscopy and Scanning Electron Microscopy. PHASE-VI EFFECT OF CADMIUM TREATED WATER ON GERMINATION OF SOYBEAN (PETRIPLATE STUDY 10 DAYS) The plants were grown in six groups as follows, and five plants were selected for checking germination parameters. GROUP-I NORMAL WATER TREATED SOYBEAN GROUP-II 5 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-III 10 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-IV 15 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-V 20 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-VI 25 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROWTH PARAMETERS CHECKED: The parameters checked on 10th day includes Germination percentage, Root length, Shoot length, Length of whole plant, Fresh mass of whole plant, Dry mass of whole plant, Dry mass / fresh mass ratio, Relative seed germination, Root length, Shoot length percentage, Germination index, Seed vigor index, Tolerance index, Germination rate percentage FINDINGS OF GERMINATION STUDY: Although the test groups showed a reduction in parameters when compared to control group, a highly significant improvement in the germination of soybean seedlings grown using with the increasing concentration of cadmium in the effluents after treatment. A significant improvement in germination percentage, root length, shoot length, fresh mass, dry mass of whole plant, relative seed germination, relative root length, shoot length percentage, germination index, seed vigor index, tolerance index and germination rate percentage were observed when compared to before treatment plants. Also a significant variation in dry mass / fresh mass ratio was observed. With these significant preliminary results obtained for treated effluents, the work was extended to red soil study using pot culture. PHASE- VII EFFECT OF CADMIUM ON METABOLISM AND ANTIOXIDANT STATUS OF SOYBEAN ON 20TH DAY AND 40TH DAY: The plants were grown in six groups as follows, and five plants were selected for checking all physical parameters and all biochemical estimations were made on three plants selected as replications. GROUP-I NORMAL WATER TREATED SOYBEAN GROUP-II 5 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-III 10 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-IV 15 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-V 20 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN GROUP-VI 25 ppm Cd CONTAMINATED ACTIVATED CARBON TREATED ARTIFICIAL EFFLUENT TREATED SOYBEAN VII.A. GROWTH PARAMETERS MEASURED ON 20TH DAY The parameters checked on 20th day includes Root length, Shoot length, Plant height, Relative root length percentage, Relative shoot length percentage, Tolerance index, Number of leaves, Trifoliate, Fresh weight, Dry weight, Dry mass/fresh mass ratio, Leaf mass ratio, Stem mass ratio, Root mass ratio, Total dry matter yield under cadmium stress, Percentage weight gain, Seed vigor index, Leaf area measurements, Leaf area index, Leaf weight fraction, Specific leaf area, Leaf area ratio FINDINGS: The following observations were made with five plants in each group grown with treated effluents, on 20th day: The growth parameters such as root length, shoot length, plant height, relative root length percentage, relative shoot length percentage, tolerance index, number of leaves, trifoliates, fresh weight, dry weight were found to be decreased when compared to control. But a highly significant improvement was seen in all these parameters, when compared with the values of plants grown with effluents before treatment. Also dry mass/fresh mass ratio, stem mass ratio, root mass ratio were also found to be varied than the before treatment plants. The other growth parameters such as leaf mass ratio, total dry matter yield under cadmium stress, percentage weight gain, seed vigor index, leaf area measurements, leaf area index, leaf weight fraction , specific leaf area, leaf area ratio were also found to be improved when compared to before treatment plants, although it is decreasing when compared to control group plants. VII.B. GROWTH PARAMETERS MEASURED ON 40TH DAY The parameters checked on 20th day includes Root length, Shoot length, Plant height, Relative root length percentage, Relative shoot length percentage, Tolerance index, Number of leaves, trifoliate, nodules per plant, Fresh weight, Dry weight of individual organs and whole plant, Dry mass/fresh mass ratio of individual organs, Leaf mass ratio, Stem mass ratio, Root mass ratio, Total dry matter yield under cadmium stress, Percentage weight gain, Seed vigor index, Leaf area measurements, Leaf area index, Leaf weight fraction, Specific leaf area, Leaf area ratio , Absolute growth rate, Crop growth rate, Unit leaf rate, Net assimilation rate, Relative growth rate, Leaf area duration FINDINGS: In the observations made with five plants in each group on 40th day, the following results were observed for the plants grown after treatment of Cd containing waste water: The growth parameters such as root length, shoot length, plant height, relative root length percentage, relative shoot length percentage, tolerance index, no of leaves, trifoliates, nodules per plant, fresh weight, dry weight of individual organs, and whole plant, leaf mass ratio, total dry matter yield under cadmium stress, percentage weight gain, seed vigor index, leaf area measurements, leaf area index, leaf weight fraction, specific leaf area, leaf area ratio were found to be increased significantly when compared with the before treatment plants. The dry mass/ fresh mass ratio, stem mass ratio and root mass ratio were also found to be varying with concentration of cadmium after treatment. The growth parameters connecting the observations made on 20th and 40th day observations such as absolute growth rate, crop growth rate, unit leaf rate, net assimilation rate, relative growth rate, leaf area duration values showed a significant improved performance when compared to the plants gr own with the cadmium contaminated waste water before treatment. VII.C. BIOCHEMICAL PARAMETERS ESTIMATED ON 20TH DAY ON ROOT SHOOT TISSUES OF SOYBEAN: The various biochemical estimations made includes, i. GROWTH PARAMETERS Chlorophyll a, b, total chlorophyll, Total carbohydrate, Starch, Total Protein ii. ENZYMES: Amylase alpha, beta and total, Glucose-6-phosphate dehydrogenase iii. STRESS MARKERS Total free amino acids, Proline, TBARS iv. NON ENZYMATIC ANTIOXIDANTS Vitamin E, Vitamin C, Reduced Glutathione v. ENZYMATIC ANTIOXIDANTS Superoxidedismutase, Catalase, Peroxidase, Glutathione Reductase, Glutathione Peroxidase. FINDINGS: The levels of growth parameters such as Chlorophyll-a, b and Total Chlorophyll were significantly found to be improved with the increase in the cadmium concentrations in the effluents after treatment. The levels of Total Carbohydrate, Starch and Total Protein were also found to be increased significantly when compared to before treatment plants in both root and leaf tissues. Although a decreasing levels of Amylase, Glucose-6-phosphate dehydrogenase were observed with increasing concentrations of cadmium, when compared to control, a significant increase in their levels was observed when compared with the plants grown before treatment in both root and leaf tissues. The stress markers like Total Free Amino Acids, Proline and Thiobarbituric Acid Reactive Substances(TBARS) were also found to be decreased in both leaf and root tissues when compared with the plants grown using effluents before treatment. The non enzymatic antioxidants such as Vitamin E, Ascorbic acid and Reduced Glutathione were found to significantly varying with the varying concentrations of cadmium after treatment. The enzymatic antioxidants such as Catalase, Peroxidase, Glutathione Reductase, and Glutathione Peroxidase were found to be significantly increased in the plants grown after treatment. Also level of Superoxide dismutase was found to be reduced than the level found in plants grown using effluents before treatment. PHASE-VIII UPTAKE AND LOCALIZATION OF CADMIUM BY SOYBEAN PLANTS BEFORE TREATMENT The level of cadmium uptake was measured by atomic absorption spectrophotometer in the tissues of root, shoot, leaves, and pod of soybean grown using Cd contaminated water before treatment. AFTER TREATMENT The level of cadmium uptake was measured by atomic absorption spectrophotometer in the tissues of root, shoot, leaves, and pod of soybean grown using Cd contaminated water after treatment. FINDINGS: The uptake and localization of cadmium levels and correlated to the results obtained in biochemical and physical parameters. Conclusions The results obtained from the present study indicates The effect of Cd on the growth, stress and antioxidant metabolism of Glycine max (L.) Merr. Plants by petriplate and pot culture studies were studied and are found to be significant. The production of low cost activated carbon from agro waste of Anacardium occidentale L.., and the removal of Cd from the waste water by the low cost AC are significant. Use of the treated water for irrigation and its effect on the growth, stress and antioxidant metabolism of Glycine max (L.) Merr. Plants by petriplate and pot culture were studied. The results may be helpful in better understanding of the various growth, metabolic and defense response mechanisms of the Glycine max (L.) Merr. plants, grown with Cd contaminated water before and after treatment using activated carbon. The effect of treated water on the growth over the non-treated water was evaluated and the results show the efficiency of the treated Cd contaminated waste water for irrigation. The results also suggest that this method may be useful as a secondary treatment method in the water treatment plants involved in treatment of sewage wastewaters. 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The Birth Of Republic By Edmund Morgan - 1440 Words

In the book, the birth of republic, Edmund Morgan showed the challenge of British taxation imposed on Americans and the quest for search of constitutional principles so as to protect their freedom leading to revolution. In this book, the author portrayed how the experience of the founders of the nation shaped the convictions and how these elements were fit into the constitution and governmental legislatures. â€Å"In 1649, as Harrington saw it, the people who owned the property of England deposed the kind and began to take charge of the government. Their action proved premature. Monarchy and Aristocracy turned out to be stronger than Harrington had supposed, but he gave the people (or at least those who owned property) the nerve to think that†¦show more content†¦In our case it was the other way round: we struck for independence. And were thereby stirred into nationality; our nation was the child, not the father, of our revolution† In the US, the weaving of ideas about constituent power and representation let, in Edmund Morgan’s phase, to ‘inventing the people’; Republican and representative government marched and hand-in-hand, although it look more than a century before the people impose themselves on the senate through seventeenth amendment . Although the predecessors in the US were English, but an increasing nationalism emerged in the US and this is prevalent in the history of Europe, Asia and Africa that the people tend to form tribes and greater communities and societies that are transformed into nations. The American civil war revolution set the foundation of the typical US liberty that resulted in the formation of the US constitution. In this book, the New Colonial Policies after 1763 were reviewed that were the main source of revolution. The revolution as portrayed in Morgan book was revolt against England whereas the internal dispute among colonies had the secondary importance as compared to revolt against England .The driving force of the revolution was to form an independent government that is free from the rule of Britain, so it was considered as a revolt against the English. There were

Ayala Museum Free Essays

Little did we know, when we were sent on a field trip to the Loyal Museum, what we would see there. All that we were told was that It was an â€Å"educational field trip† and something to do with Flanagan. Net’s advocacy of anything and everything Filipino. We will write a custom essay sample on Ayala Museum or any similar topic only for you Order Now So, we were really in for a treat when we set foot on the fourth floor of the museum and a short film started to play the moment we stepped inside the sliding doors. â€Å"Gold of Ancestors: Crossroads of Civilization†, it began, and went on to show some gold artifacts that had been found on archaeological digs all over the country. We were mesmerism’s from the very first one. Then, Just beyond the small chamber where we were viewing the film, we caught sight of the display cases containing the very artifacts that were now flashing on the screen. After that, we could hardly Walt to go and see them up close We saw golden bangles, rings, and necklaces, earrings, tiaras and studs, belts, bowls, cups and hilts of weapons. I was fascinated when I saw the burial masks, with stylized features engraved In the gold. I thought: forget ancient Egypt, It turns out the Philippines has its own! Aside from the burial masks, there were also â€Å"orifice coverings†, shaped like the eyes, the nose and the mouth. I guess If the dead couldn’t afford a whole mask, they could just go for the important parts! Whew! Move over, King -rut! Aside from the burial masks, I was also fascinated with the Kina, a small vessel shaped like a mythical woman-bird, who appeared to be holding some kind of Jug. Her eyes looked serenely out at the people who had come to see her. There was also the bluntly, an anthropomorphic plaque depleting the head of a lady with what seemed like an elaborate hairdo or headdress. I also drooled over a cuff or wrist ornament, as wide as a sleeve and intricately worked, so that the gold mesh was like lace. The information on it said it weighed 204 grams. Heavy! There were also earrings so big and so heavy that we all wondered how the owner could possibly have worn It without getting his or her ears torn. My personal favorite, however, was a beautiful belt (l know someone’s snickering while reading TLS! ). Catalog No. 81. 5175, weighing 575. 1 g, measuring 68. Xx CM. Circa 10th 13th century. It was made of fine mesh, with a zigzag design running along its length, and two golden plaques ornamented with granules of gold painstakingly arranged into stylized designs. Oh, I could have stood there and stared at it forever! Then, there was the piece De resistance: the UAPITA or Sacred Thread, weighing almost 4 kilos (yeah- peeve! ), strikingly similar to one depicted In the 16th century Boxer Codex, said to be the earliest description of the people of the Philippines In a Western language. Copy of which can be found in the exhibit. The UAPITA was supposed to be draped around the neck then attached to the wrist. Wow, the person who wore that must’ve been pretty strong! What was striking about these artifacts was that they seemed so Pinto to me. There were at least two necklaces or bracelets that were woven banging- Tyler: take four flat strips of gold and weave a fifth one back and forth across it, the reminded me of the legs of a modern armchair made of into because it h ad the same basket-weave technique. Some of the other items were decorated simply by poking holes in the gold to create a lacy pattern. The tiaras were created by cutting a sort of fringe in a strip of gold, then bending them back and forth to make a crown-like design. Seeing the exhibit made all of us walk a little taller and prouder. We had always read about the treasures of other lands, like Egypt and Troy; now we know that we have our own! Mayhap nag Philippians! How to cite Ayala Museum, Papers

Diet Analysis Essay Example For Students

Diet Analysis Essay Since my childhood, I have always had an aggressive appetite, and obviously, not all the meals I ate were healthy and nutritious. For a long time, I did not care much about what I ate and how my food choices and eating habits influenced my health. However, recently, I have started to pay more attention to my daily diet. In particular, I decided to track my daily meals for a week, write down everything I eat and prepare my diet analysis based on calorie intake and the amount of protein, fats, and carbohydrates consumed. It has helped me find out what my organism lacks and, on the contrary, what is redundant. While analyzing what the foods I typically eat every day are and how my eating choices change over the days, I have found out that there are some negative tendencies in my daily diet that I definitely need to change. At the same time, there are also certain eating habits that need to be further encouraged and maintained. What have I found out? First of all, my diet analysis has revealed that I consume not enough water. I thought that I drink much, so the result was a shock to me. Over the days, I systematically drank less than 1 l of plain water or a bit more. The rest of the consumed liquid was mainly tea or coffee. I know that it is important to drink more water as water can help with hair and skin. Besides, in general, drinking water makes the body work properly. If I want to make a change in my daily diet, I definitely need to increase the amount of plain water drunk every day and eliminate the amount of coffee, juices, and sodas consumed. Speaking about my overall calorie intake, it was rather unstable during the week of my diet analysis. While on some days, it was normal, on other days, it was either excessive or insufficient. For example, on Monday, my calorie intake was 648.5, while on Sunday, it was 2343.6. The matter is that on working days, I often have no time for eating. Sometimes, I choose eating at fast foods, although I know that such eating habits can lead to severe health problems. At the weekends, I have more time and can prepare meals by myself. It is important to stabilize my calorie intake and consume the required number of calories to keep my body in shape and not put on weight. Apart from the need to stabilize calorie intake, my diet analysis has also revealed some other issues. I understand that tracking my eating habits just for one week is not enough to make conclusions as over the days; my eating choices can significantly change. Still, my diet analysis can help me identify the negative tendencies and try to establish a healthier diet on a daily basis. Vitamins as integral part of the healthy life An important aspect of my diet analysis research was vitamin intake and my vitamin D level in particular. After I had reviewed my vitamin intake, I understood that the foods I ate did not include many vitamins. My vitamin D level is particularly low. It can be understood because it is winter now, and that is why my exposure to sun is low. However, not only the change in the weather can help increase my vitamin D level. One of the main sources of vitamin D is milk, and I almost never drink milk. As I do not have any allergies, I can include milk to the foods I consume every day. My favorite drink is coffee, and sometimes, I drink it too often. Instead, I can drink milk or water. Besides that, there is a possibility to eat vitamin D supplements to increase my vitamin D level. I realize that I need to do something about my eating choices and eating habits to ensure the required level of vitamin D in my organism. .u09604941c3b0e72000fff27eca49c0e1 , .u09604941c3b0e72000fff27eca49c0e1 .postImageUrl , .u09604941c3b0e72000fff27eca49c0e1 .centered-text-area { min-height: 80px; position: relative; } .u09604941c3b0e72000fff27eca49c0e1 , .u09604941c3b0e72000fff27eca49c0e1:hover , .u09604941c3b0e72000fff27eca49c0e1:visited , .u09604941c3b0e72000fff27eca49c0e1:active { border:0!important; } .u09604941c3b0e72000fff27eca49c0e1 .clearfix:after { content: ""; display: table; clear: both; } .u09604941c3b0e72000fff27eca49c0e1 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u09604941c3b0e72000fff27eca49c0e1:active , .u09604941c3b0e72000fff27eca49c0e1:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u09604941c3b0e72000fff27eca49c0e1 .centered-text-area { width: 100%; position: relative ; } .u09604941c3b0e72000fff27eca49c0e1 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u09604941c3b0e72000fff27eca49c0e1 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u09604941c3b0e72000fff27eca49c0e1 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u09604941c3b0e72000fff27eca49c0e1:hover .ctaButton { background-color: #34495E!important; } .u09604941c3b0e72000fff27eca49c0e1 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u09604941c3b0e72000fff27eca49c0e1 .u09604941c3b0e72000fff27eca49c0e1-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u09604941c3b0e72000fff27eca49c0e1:after { content: ""; display: block; clear: both; } READ: The Lottery Persuasive EssayWhile working on my diet analysis project, I realized that I even though I lead an active lifestyle, I do not exercise enough. Bringing more exercising into my life will definitely help me to stay in shape. In particular, I am going to add jogging to my daily routine. As the sun is the source of vitamin D, jogging will also help me to increase my vitamin D level. My research met the results My diet analysis research has shown that in general, my vitamin levels are very low. It can be explained by the fact that I eat not many vegetables and fruit. That is why based on my research; I decided to make a change in my daily diet and add more vegetables and fruit to the foods I eat. I believe that it will also help regulate my calorie intake at large. In particular, my daily diet includes almost no fruit. For example, over the days, I ate fruit just a few times. On Thursday, I drank a smoothie, and the only fruit I ate that day were the ones added to this smoothie. Another significant problem in my diet is eating too much fat. As during the week, I often eat at fast foods, it means that I eat the foods that contain trans-fat and saturated fat almost every day. For example, my overall intake of saturated fat over a week was 28.7, and this number is huge. I need to exclude fast food from my daily diet as eating too much trans fat, and saturated fat can not only lead to obesity but also provoke serious health problems. To sum up, I should say that my diet analysis research has turned out to be very helpful and useful for me. With its help, by tracking my eating habits and the foods I eat for a week, I identified the key problems in my daily diet and made some significant decisions. For instance, I found out that I drink not enough water. I decided to work on it and develop a habit of drinking the required amount of plain water every day instead of drinking too much coffee. Water is essential for the overall health and well-being, and thus, drinking enough water will be my priority. Besides that, I understood that my vitamin D level is rather low. I will do everything to increase this level by drinking milk and eating other foods that contain vitamin D. Also; my diet analysis showed that all my vitamin levels are low because of consuming not enough fruit and vegetables. I do not like fruit very much, but I will try to add as many of them as is needed for my daily diet to stay healthy and ensure vita min intake. What is more, I eat too much trans-fat and saturated fat, and I want to change my eating habits and eliminate the number of fats consumed in my daily diet.